Extended Data Fig. 1: Nitrate dynamics of chloramphenicol untreated (CHL−) and treated (CHL+) conditions in the dataset.
From: Functional regimes define soil microbiome response to environmental change
Time series measurements of nitrate in chloramphenicol-untreated (CHL−, black points) and treated (CHL+, red points) across 4 days are shown. Each row is from the identical soil sample of a native pH level (\({{\rm{pH}}}_{{{\rm{H}}}_{2}{\rm{O}}}\)), indicated at the right end of each row in the order of most acidic (top) to most basic (bottom). Each row has 13 columns, which are the 13 different levels of short-term pH perturbations. The targeted perturbed pH levels were determined by constructing a soil pH titration curve before the experiment and computing how much acid (HCl) or base (NaOH) to add to the slurries. Perturbed pH levels are indicated inside each panel, which are obtained by measuring the stabilized pH values at the endpoint of the experiment (see Methods). Each line connects the points of measurements of a replicate, constituting the 3 replicates per perturbed condition. The pink-colored box for each row indicates the condition without any acid/base addition, where the pH of these conditions also changes with incubation. The total number of independent biological replicates in the slurry experiment is n = 1,704, including no-nitrate (n = 120) and cycloheximide controls (n = 120). Soil 19 and Soil 20 are not shown due to having different numbers of perturbed pH levels (7 and 3, respectively). CHL+/− slurry experiments (n = 1,404) from Soil 1–18 (18 soils × 13 perturbations × 2 CHL+/− × 3 biological replicates) are shown, totaling 14,011 nitrate time-series data points after excluding 29 data points due to experimental errors.
