Fig. 4: Proof of directional rotation for motor 3a.
From: Redox-powered autonomous directional C–C bond rotation under enzyme control
a, Oxidation of (Sa)-D2-3a ((Sa)-D2-3a (10 mM, >98% ee), ADH 291 (7 U ml−1), NADP (1 mM, 10 mol%), YcnD (12 μM), O2 (air), NaPi (100 mM, pH 7), DMSO (10% v:v), 40 °C, 48 h), analysis of deuterium distribution at aldehydic CDO and benzylic CD2OH positions and example determination of rox(Sa)/rox(Ra). b, Oxidation of (Ra)-D2-3a ((Ra)-D2-3a (10 mM, >98% ee), ADH 291 (7 U ml−1), NADP (1 mM, 10 mol%), YcnD (12 μM), O2 (air), NaPi (100 mM, pH 7), DMSO (10% v:v), 40 °C, 48 h) and analysis of deuterium distribution at benzylic CD2OH and aldehydic CDO positions. c, Histogram illustrating the distribution of the net angles of rotation of 107 simulated molecules of 3a after 48 h of operation (see Supplementary Information Section 11 for details).
