Extended Data Fig. 9: Generated Cas9-like proteins are less immunogenic than SpCas9. | Nature

Extended Data Fig. 9: Generated Cas9-like proteins are less immunogenic than SpCas9.

From: Design of highly functional genome editors by modelling CRISPR–Cas sequences

Extended Data Fig. 9: Generated Cas9-like proteins are less immunogenic than SpCas9.The alternative text for this image may have been generated using AI.

iELISA antibody quantification values indicate raw OD450nm values showing the amount of bound human antibody to each Cas9 protein. Plates were coated with purified proteins for SpCas9, PF-CAS-182 (OpenCRISPR-1), PF-CAS-189, and PF-CAS-151 at a concentration of 1 µg/mL (100 ng/well). Serum samples were diluted at 100-fold to 1600-fold. Generated Cas9-like proteins are less immunogenic than SpCas9 at one or more sample dilution levels.

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