Fig. 1: Multiregional recordings during MPOA-driven consummatory behaviours.
From: A circuit that integrates drive state and social contact to gate mating
a, Representative image of ChR2 expression in MPOA during MPOAEsr1∩Vgat optogenetic stimulation. b, Schematic (top) and raster plots (bottom) of MPOAEsr1∩Vgat optogenetic stimulation induced behaviours towards a female. Bil., bilateral; Con, Cre-ON; Fon, Flp-ON. c, Percentage of MPOA-stimulation (ON) or sham (OFF) trials resulting in mounting with USVs towards a female, male or inanimate object (n = 8 mice, 6 trials per mouse).Stim, stimulation. d, Percentage of MPOA-stimulation trials with mounting under white light (A), dark infrared light (B), whisker cuts/whiskerless (C) or face numb and whiskerless (D) conditions (n = 5 mice, 20 trials per mouse). e, Probability of mounting with MPOA stimulation delivered before (orange) or during object contact (grey; n = 5 mice, 10 trials per mouse). f, Schematic of the experimental model. g, Probability of USV+ bilateral reach (left) and run (middle) in MPOA stimulation alone, contact alone or both (stimMPOA + contact) conditions (n = 5 mice, 10 trials per mouse). Number of USVs per trial (right). h, Representative image of MPOAEsr1∩Vgat axons labelled with ChR2-eYFP. MRN, midbrain reticular nucleus; PAG, periaqueductal grey; RN, red nucleus; SN, substantia nigra. i, Schematic of probe trajectories. Opto, optogenetic. j, Spike rasters for four single units under the three conditions, ten trials per condition. FR, firing rate; RRF, retrorubral field; vPAG, ventral periaqueductal grey. k, Percentage of multimodal cells with significantly higher (right, more than 2σ) or lower activity (left, less than −2σ) under the multimodal condition relative to unimodal (n = 2,300 total single units from 14 mice; 10 trials per condition). Dashed line, mean of all cells. dPAG; dorsal periaqueductal grey. l, Mean population firing rate across each brain region during the three conditions. norm., normalized. *P < 0.05, **P < 0.01, ***P < 0.001. For all figure panels, data are mean ± standard error of the mean (s.e.m.). See Supplementary Table 2 for further details of the statistical analyses. Scale bars, 0.5 mm (a,h).
