Extended Data Fig. 3: Primary tumor burden of MMTV-Her2 and IL-6 KO/MMTV-Her2 mice and further characterization of IL6KO metastasis post IAV.

Kaplan-Meier curve for time to sacrifice due to primary tumor burden of MMTV-Her2 and IL-6 KO/MMTV-Her2 mice (n = 4/group) (a). Mice were sacrificed when primary tumors reached 2 cm in diameter; there was not a significant difference between the two groups. Quantification of PyMT⁺ lesions ( ≤ 5 cells) with at least one Ki67⁺PyMT⁺ cell in lungs 21 dpi with IAV (b) (n = 3/group, n = 4 for PBS). PyMT⁺ micrometastases (defined by lesions with an area <0.03 mm²) were quantified (c) (n = 7 WT, 4 IL6^–/–); the WT groups are the same as in Fig. 1f, and are included to allow comparison with the IL-6 KO. EO771 cells (2 × 10⁵) were implanted into WT or (n = 3) IL-6 KO C57BL/6 mice (n = 4), infected with IAV, and harvested at 12 dpi, stained with H&E, and tumor area and the numbers of lesions quantified (d and e). Significance is determined by one-way ANOVA test (b,c) or two tailed Student’s t test (d,e). Size of mammospheres from MMTV-Her2 mice per well 72 h post daily treatment with either PBS (CTRL) or IL-6 (10 ng/mL) (f). Size of EO771 derived organoids per well 72 h post daily treatment with either PBS (CTRL) or IL-6 (10 ng/mL) (g) (n = 5/group). Significance by Mann-Whitney tests. All box-and-whisker plots are presented as maximum value (top line), median value (middle line), minimum value (bottom line) with all data points shown (dots).

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