Extended Data Fig. 6: Orthogonal GCSFR drives myeloid-like T cell states.
From: Expanding the cytokine receptor alphabet reprograms T cells into diverse states
a, Proportion of each treatment group unmapped to the reference atlas of TILs29. b, Gene Ontology enrichment analysis of oGCSFR-upregulated genes identified the top 20 enriched pathways (enrichment score ≥1.2, adjusted p < 0.05). c–f, NT pmel CD8+ T cells and those transduced with Nfic–Maf–MafB, or oGSCFR were cultured for 72 h, with oGSCFR pmel CD8+ T cells stimulated with MSA-oIL2 (500 nM) during this period, followed by flow cytometry analysis (n = 7 biological independent samples). Shown are the frequencies of Mac-1+ (c), CD14+ (d), CD64+ (e), and Gr-1+ (f) cells. g, MFI of pHrodo Red E. coli bioparticles (n = 3 biologically independent samples). h, Shown are the pHrodo Red confluences (n = 8 biologically independent samples). i, The experimental setup is described in Fig. 4l. Shown are the representative fluorescence images of GFP+ L. monocytogenes at the highest concentration. j, Experimental setting is described in Fig. 4n,o. Representative fluorescence images of CSFE+ (green) effector cells cocultured with FarRed+ (red) A20 target cells at a 1:1 E:T ratio. k, Pmel CD8+ T cells were cocultured for 2 h with FarRed+ A20 cells pretreated with mouse CD19 (mCD19) antibody, followed by flow cytometry analysis (n = 6 biologically independent samples). Shown are the frequencies of FarRed+CD8+ cells. l, oGCSFR pmel CD8+ T cells were preserved in MSA-oIL2 (500 nM) for 24 h. B16F10-mCD19 cells were co-cultured with pmel CD8+ T cells at a 1:1 E:T ratio with MSA-hoIL2 (200 nM), in the presence or absence of mCD19 antibodies for 48 h. Cells were collected for flow cytometry analysis (n = 4 biologically independent samples). Shown are the percentages of B16F10-mCD19 cell killing. All data represent mean ± s.e.m. and are analysed by two-tailed Student’s t-test (k) or one-way ANOVA with Tukey’s post-test (c–f, g, l).
