Extended Data Fig. 10: Goblet cell-specific mAChR4 ablation enhanced MASLD development in mice.
From: mAChR4 suppresses liver disease via GAP-induced antimicrobial immunity
(a–i) WT (n = 12) and Chrm4ΔGC (n = 14) littermate mice were fed Western diet, together with glucose (18.9 g L−1) and fructose (23.1 g L−1) in the drinking water (Western diet; WD) for 24 weeks; 11 independent experiments. (k–s) Same protocol was followed for mAChR4Act/GC mice treated with vehicle (n = 12) or 400 μg kg−1 DCZ (n = 6); 6 independent experiments. (a, j) Body weight increase (left panels), area under the curve (AUC) of the body weight increase (right panels). (b, k) Cumulative food intake. (c, l) Cumulative liquid intake. (d) Liver weight at 24 weeks. (e, m) Plasma ALT. (f) Hepatic triglycerides. (g, n) Representative images of H&E-stained liver tissue. Scale bar=100 μm. (h, q) Quantification of Sirius red-stained liver area. (i, r) Representative images of Sirius red-stained liver tissue. Scale bar=100 μm. (o, p) Hepatic Cd14 and Timp1 mRNA levels. Diagonal ticks indicate a break in the y-axis scale (e). P values were determined using two-way ANOVA with BKY FDR test (a–left panel, b–c, j–left panel, k–l), or either the two-sided unpaired Student t-test (d, f, h, j-right panel, q), unpaired Welch’s test (m, o–p) or Mann-Whitney U (a–right panel, e) test. P (or q) *< 0.05, **< 0.01, ***< 0.001, ****< 0.0001. Q when applying the BKY FDR method.
