Extended Data Fig. 5: The spatial distribution of SSU pre-rRNAs in H9, D30 arcuate neuron and SH-SY5Y cells.
From: Pre-rRNA spatial distribution and functional organization of the nucleolus
a. 5′ ETS-1 localization in the DFC region remains consistent across different cell types. Left, schematic of the 5′ ETS-1 probe position. Middle, representative single-slice and averaged SIM images of 5′ ETS-1 (magenta) and FBL (green) in the indicated cell types. Right, the corresponding line intensity plots of 5′ ETS-1 and FBL. n = 51, 12, 25 DFC units in 15 (H9), 10 (D30 arcuate neuron), 9 (SH-SY5Y) cells, respectively. b. ITS1 localization in the DFC–PDFC regions remains consistent across different cell types. Left, schematic of the ITS1 probe position. Middle, representative single-slice and averaged SIM images of ITS1 (magenta) and FBL (green) in the indicated cell types. Right, the corresponding line intensity plots of ITS1 and FBL. n = 48, 27, 52 DFC units in 10 (H9), 12 (D30 arcuate neuron), 16 (SH-SY5Y) cells, respectively.
