Extended Data Fig. 8: Electrophysiology of SCLC cells.
From: Functional synapses between neurons and small cell lung cancer
a) Example of a patched DsRed-expressing SCLC cell (COR-L88) under whole-cell configuration in cortical neuron-SCLC co-cultures. b) Whole-cell, voltage-clamp traces of sPSCs in SCLC cells (COR-L88) in the presence or absence of neurons. c) Quantification of sPSC frequency in co-culture in the presence or absence of the indicated blockers (TTX, CNQX, D-AP5, Riluzole and Bicuculline) (n = 7-30 cells per condition). All conditions are compared to untreated co-cultures. q values: two-sided Mann-Whitney with FDR correction d-g) Whole-cell voltage-clamp traces of H524 cells d) Traces recorded at three different voltages (−70 mV, 0 mV, and +40 mV) in mono-culture. e) Traces recorded at +40 mV in co-culture with cortical neurons. The synaptic events (red stars and numbers) can be completely abolished by the application of the NMDA receptor blocker D-AP5 and display a long decay time lasting several hundred milliseconds. f) Traces recorded at −70 mV and 0 mV in co-culture with cortical neurons. Note the occurrence of synaptic events at 0 mV, indicating a GABA-A-mediated chloride inward current. g) Traces recorded at +40 mV in co-culture with Channelrhodopsin 2-eYFP expressing (ChR2-eYFP, green) cortical neurons after a short blue light pulse (5 ms). Note the partial decrease in event amplitude during NMDA receptor blockade with D-AP5 (orange), followed by complete abolishment after additional GABA-A receptor blockade with Gbz (lower trace). h) Whole-cell, voltage-clamp recording in an acute hippocampal slice of grafted DsRed-expressing murine SCLC cells. i) Quantification of sPSCs in grafted cancer cells in acute slices in the absence or presence of the indicated blockers (TTX, CNQX, D-AP5 and Bicuculline). All conditions are compared to untreated slices. q values: two-sided Mann-Whitney with FDR correction, n = 8-17 cells per condition.
