Extended Data Fig. 2: The full-length prefusion structure of gB.
From: A nanobody specific to prefusion glycoprotein B neutralizes HSV-1 and HSV-2
(a) Processing pipeline, outlining individual steps with particle numbers and options used deviating from default settings. Except initial steps using WARP51, all steps were done using CryoSPARC52. (b) 2D classes showing the 10 most populated classes. (c) Fourier shell correlation (FSC) for the density map corresponding to the prefusion structure is shown. Resolution was calculated using an FSC threshold of 0.143. (d) Final map coloured by local resolution (FSC threshold 0.143) using a range of 1.5–4.5 Å.
