Extended Data Fig. 8: PD-1 checkpoint blockade at late time point similarly disrupts accumulation of high affinity SLAMF6+TSL in the tdLN.
From: Inhibitory PD-1 axis maintains high-avidity stem-like CD8+ T cells
a, Experimental scheme illustrating the checkpoint treatment strategy of late PD-1 ligand blockade initiated at Day 10. b, Representative flow cytometry plots of TCF-1 and SLAMF6 expression on OVA-tetramer+ CD8+ T cells in the tdLN at Day 19.5 and the gating strategy defining the three major TSL and TEFF subsets. The total numbers of each subset recovered from the tdLN are shown in (c,d). e, Total number of “high affinity” SLAMF6+ TSL (imputed affinity index >mean of control (PBS-treated) SLAMF6+ TSL from the same experimental cohort) in the tdLN. Standardized log transformed imputed TCR affinity index (f) and mean imputed affinity index (g) of each treatment group, normalized to PBS-treated SLAMF6+ TSL from the same experimental cohort. h, Fraction of “high affinity” SLAMF6+ TSL derived from the quantification of cell numbers in (e) divided by total SLAMF6+ TSL count (d). Data pooled from 3 independent experiments (n = 10 (PBS-treated) and n = 12 (aPD-L1/2-treated)). Error bars: means ± s.d. *p < 0.05, ****p < 0.0001; unpaired two-tailed t-test.
