Fig. 2: PD-1+SLAMF6+ TSL cells are uniquely retained in the tdLN.
From: Inhibitory PD-1 axis maintains high-avidity stem-like CD8+ T cells
a–c, Optically cleared tdLN slice from a day 8 tumour-bearing mouse without adoptive transfer of transgenic T cells. Activated polyclonal CD8+ T cells are gated based on co-expression of CD8b and Ki-67. a, Day 8 inguinal draining lymph node kernel density map showing foci of activated polyclonal CD8+ T cells. The density was estimated using a Gaussian kernel of 6 µm bandwidth, weighted on normalized PD-1 expression. The green box indicates the region magnified in b, in which T cell–cDC1 cell clusters are further enlarged and displayed with individual protein markers: C1 (c) and C2 (Extended Data Fig. 3b). Data are representative of two independent experiments. n = 5. Scale bars, 50 μm (b) and 20 μm (c). d,f, Representative gating strategy of OVA–tetramer+CD8+ T cells recovered from tissues collected from day 10 tumour-bearing mice and analysed using flow cytometry, based on TCF-1 and PD-1 (d) or SLAMF6 (f). e,g, Quantification of the proportions of PD-1+ (e) and SLAMF6+ (g) cells of the TCF-1+ TSL and TCF-1− Teff cell subsets. Data are pooled from two independent experiments. n = 6 (tdLN, spleen, tumour) and n = 3 (contralateral brachial lymph node (contra-bLN)). Data are mean ± s.d. Statistical analysis was performed using unpaired two-sided t-tests; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.
