Extended Data Fig. 10: Biochemical and physiological effects of dietary serine/glycine restriction in mice.
From: Rewiring of cortical glucose metabolism fuels human brain cancer growth
a, Abundance of serine in plasma from intracranial GBM-bearing mice fed either control diets or serine/glycine-restricted diets for 4 weeks. Data are mean ± s.e.m., and groups were compared by t-test. n = 21–25 mice bearing intracranial HF2303, GBM38 or GBM12 tumours per group from 5 independent experiments. b, Weights of mice fed control diets or serine/glycine-restricted diets. Data are mean ± s.e.m., and differences between control diet groups and serine/glycine-restricted diet groups for each sex were compared by t-test with Holm-Šídák correction. n = 5 mice per group. *p ≤ 0.05; **p ≤ 0.01, ***p ≤ 0.001. c-x, Complete blood counts (c-r) and serum chemistries (s-x) were analysed for C57BL/6 J mice fed either a control diet or serine/glycine-restricted diet for 9 months. c, Lymphocyte percentage of white blood cells and lymphocyte counts. d, Monocyte percentage of white blood cells and monocyte counts. e, Neutrophil percentage of white blood cells and neutrophil counts. f, Eosinophil percentage of white blood cells and eosinophil counts. g, Basophil percentage of white blood cells and basophil counts. h, White blood cell counts. i, White blood cell distributions. Data are mean ± s.d. j, Red blood cell counts. k, Platelet counts. l, Haemoglobin concentrations. m, Haematocrit. n, Mean corpuscular volume. o, Mean corpuscular haemoglobin. p, Mean corpuscular haemoglobin concentration. q, Mean platelet volume. r, Red blood cell distribution width. s, Creatinine. t, Blood urea nitrogen. u, Albumin. v, Sodium. w, Potassium. x, Chloride. c-x, Lines represent means. Groups were compared by t-test with n = 6 mice per group except for albumin measurements in panel u (n = 2–3 mice per group), in which experimentation was limited by sample volume and no statistical comparison could be made. Abbreviations: LYM (lymphocyte), MONO (monocyte), NEU (neutrophil), EOS (eosinophil), BAS (basophil), WBC (white blood cell), RBC (red blood cell), PLT (platelet), HGB (haemoglobin), HCT (haematocrit), MCV (mean corpuscular volume), MCH (mean corpuscular haemoglobin), MCHC (mean corpuscular haemoglobin concentration), MPV (mean platelet volume), RDW (RBC distribution width), CREA (creatinine), BUN (blood urea nitrogen), ALB (albumin), Na (sodium), K (potassium), Cl (chloride).
