Extended Data Fig. 4: Spatially defined isotope labelling in cortex and GBM.
From: Rewiring of cortical glucose metabolism fuels human brain cancer growth
a, H&E staining of brains from orthotopic GBM bearing mice intraperitoneally injected with either vehicle (left) as a negative control or [U13C]glucose (right). b-h, MALDI-MS was used to determine 13C enrichment of lactate (b), malate (c), aspartate (d), GABA (e), glutamate (f), glutamine (g), and AMP m + 5 (h). Tissue maximum is set to 100%. i-k, Tissues resected from brain cancer patients receiving either [U13C]glucose infusion (patients 1–8) or no infusion (UL 1–2) were assessed by MALDI-MS for 13C isotope labelling of malate (i), glutamate (j), and glutamine (k). Colour bar maximum set at true enrichment. l-n, Quantification of 13C-labelling of malate (l), glutamate (m), and glutamine (n) for all data points (pixel values) from spatial MALDI-MS scans shown in panels i-k and Fig. 2e. In box plots, centre line represents median, box limits represent upper and lower quartiles, and whiskers show 1.5x interquartile range. Outlier points are hidden. Violin plots are trimmed to the range of the data. All violins have the same maximum width. For a and b, the images of 13C-labelled tissues are also shown in Fig. 1g. For c, the image of 13C-labelled tissue is also shown in Fig. 2f. For h, the image of 13C-labelled tissue is also shown in Fig. 2i. For i, the images corresponding to patient 7 are also shown in Fig. 2e. Abbreviations: UL (unlabelled).
