Extended Data Fig. 5: G-CSF-administered grafts harbour HSCs with normal functions.

a, Mean fluorescence intensity (MFI) of cKIT, CD150 and CD41 in HSCs from the host femurs and the grafts in the experiment shown in Fig. 1a. 8 host femurs and 8 grafts from 8 G-CSF-administered host mice. b, Quantification of mRNA levels of the indicated cell cycle regulators in HSCs from the host femurs and the grafts. 8 host femurs and 8 grafts from 8 G-CSF-administered host mice. c, Blood chimerism (CD45.2) in myeloid (CD11b⁺), B (B220⁺) and T (CD3ε⁺) cells of recipient mice transplanted with HSCs (CD45.2) from G-CSF-administered host femurs or grafts in competition with CD45.1⁺ BM cells at the indicated timepoints after primary HSCT in the experiment shown in Fig. 1a. n = 10 mice per group. d, e, BM chimerism in whole BM, myeloid, B, T cells (d) and HSCs (e) at 5 months after primary HSCT. n = 10 mice per group. f, Blood chimerism (CD45.2) in total WBC, myeloid, B and T cells of recipient mice at the indicated timepoints after secondary BMT. n = 10 mice per group. g, BM chimerism in whole BM, myeloid, B and T cells at 5 months after secondary BMT. n = 10 mice per group. Data are mean ± s.e.m. For box plots, the box spans from the 25th to 75th percentiles and the centre line is plotted at the median. Whiskers represent the minimum to maximum range. Significance was assessed using a two-tailed unpaired Student’s t-test.

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