Extended Data Fig. 9: HSC numbers are restricted at both systemic and local levels.
From: Haematopoietic stem cell number is not solely defined by niche availability
a, b, The number of ECs (a) and MSCs (b) in the non-targeted bone (pelvis) after localized irradiation in the experiment shown in Fig. 3a. n = 6 mice per group. c, Quantification of mRNA levels of the indicated HSC niche factors in MSCs from the non-targeted bone (pelvis) after localized irradiation. n = 6 mice per group. d, CXCL12 and SCF levels in BMEF of the non-targeted bone (pelvis) measured by ELISA after localized irradiation. n = 6 mice per group. e, HSC numbers in the indicated bones and the spleens after localized irradiation. The number in parentheses indicates the number of bones examined per mouse. n = 6 mice per group. f, Schematic of parabiosis experimental design. The diagram was created using BioRender. Takeishi, S. (2025) https://BioRender.com/9d3nv16. g, Percent partner-chimerism in parabionts. n.a.: not available due to expression of the same CD45 isoform. n = 8 mice per group. h, i, HSC numbers per femur (h) and spleen (i) from mice of the indicated genotypes. 8 mice and 8 partners from 8 parabionts per group. j, HSC numbers in the entire bodies per parabiont of the indicated genotypes. n = 8 parabionts per group. Data are mean ± s.e.m. Significance was assessed using a two-tailed unpaired Student’s t-test (a-e) or one-way ANOVA (h-j).
