Fig. 3: HSC numbers are limited locally.
From: Haematopoietic stem cell number is not solely defined by niche availability
a, The experimental strategy and analyses for localized irradiation to limbs at a dose of 20 Gy. b,c, The number of ECs (b) and MSCs (c) in the targeted bones (limbs) after localized irradiation. n = 6 mice per group. d, HSC numbers in the targeted bones (four limbs), non-targeted regions (skull, spine, rib cage, pelvis, spleen and liver) and the sum of HSCs after localized irradiation. n = 6 mice per group. e, Schematic of the transplantation of a single WT femur into Cdh2-creER;Cxcl12fl/fl mice and analyses. i.p., intraperitoneal. f, HSC numbers per host femur and graft of the indicated genotypes. 8 femurs from 8 sham-operated mice, 8 host femurs and 8 grafts from 8 bone transplantation hosts in both Cxcl12fl/fl and Cdh2-creER;Cxcl12fl/fl groups. g–i, HSC numbers in the spleens (g) and in the entire body of hosts (excluding grafts) (h), and the sum of HSCs in the hosts and the grafts (i) of the indicated genotypes. n = 8 mice per group. Data are mean ± s.e.m. Significance was assessed using two-tailed unpaired Student’s t-tests (b–d) and one-way ANOVA (f–i). The diagrams in a and e were created using BioRender. Takeishi, S. (2025) https://BioRender.com/9d3nv16.
