Fig. 4: Homeostatic mechanism allowing for HSC replenishment.
From: Haematopoietic stem cell number is not solely defined by niche availability
a, Schematic of six WT femur transplantation into SCF-deficient mice. b, HSC numbers per host femur and graft of the indicated genotypes. n = 8 femurs from 8 sham-operated mice, 8 host femurs and 48 grafts from 8 hosts in both Kitlfl/fl and Cdh2-creER;Kitlfl/fl groups. c,d, HSC numbers in the entire body of hosts (c) and the sum of HSCs in the hosts and the grafts (d) of the indicated genotypes. n = 8 mice per group. e, Schematic of G-CSF administration, followed by splenectomy and BMT. f, HSC numbers in the blood on the last day of G-CSF injection. n = 12 and 6 mice, respectively. g, HSPC numbers in the blood at 1 week after G-CSF administration. n = 12 and 6 mice, respectively. h,i, The HSC frequency (h) and numbers (i) in the femurs of the indicated cohorts at 2 months after G-CSF administration. n = 6 mice per group. j, WBC chimerism (CD45.2) in recipient mice transplanted with BM cells (CD45.2) from the indicated cohorts mixed with competitor BM cells (CD45.1). n = 10 mice per group. k, HSC numbers in the entire body of the indicated cohorts at 2 months after G-CSF administration. n = 6 mice per group. l, Schematic of WT femur transplantation and HSPC transfer into SCF-deficient mice. m, HSC numbers per host femur and graft of the indicated genotypes and conditions. n = 8, 8, 8, 8, 8, 8, 48, 8 and 48 bones, respectively. n, The sum of HSC numbers in the hosts and the grafts of the indicated genotypes and conditions. n = 8 mice per group. Data are mean ± s.e.m. Significance was assessed using two-tailed unpaired Student’s t-tests (f and g) or one-way ANOVA (b–d, h–k, m and n). The diagrams in a, e and l were created using BioRender. Takeishi, S. (2025) https://BioRender.com/9d3nv16.
