Extended Data Fig. 11: Analysis of partial degradation of DNA2dd and expression of patient-derived DNA2 mutant L48P.
From: DNA2 enables growth by restricting recombination-restarted replication
a, DNA2 immunoprecipitation and western blot (representative of two independent experiments) on lysates from RPE-1 OsTIR1 DNA2dd cells treated with doxycycline (1 μg/ml) and the indicated concentrations of IAA for 24 h. KU80, input control. b, RPE-1 OsTIR1 DNA2dd cells treated as in panel a and analysed for micronuclei after three days (n = 3 technical replicates). Data presented as mean values ± s.d. c, Analysis of RPA foci in RPE-1 OsTIR1 DNA2dd cells complemented or not with doxycycline-induced DNA2 L48P as indicated after treatment with DIA for 16 h and pulsed with EdU (10 μM) for the final 60 min. Cell-cycle stage was determined by DAPI intensity and EdU incorporation into replicating cells. Data is presented as mean values ± s.d. (n = 3 independent experiments).
