Extended Data Fig. 8: Further analysis of DNA methylation and RNA expression in E11 facial and forebrain regions (pixel size, 10 μm).
From: Spatial joint profiling of DNA methylome and transcriptome in tissues
a, Spatial distribution of all clusters for DNA methylation (left) and RNA transcription (right). b-c, Integration of Spatial-DMT RNA data with scRNA-seq data from mouse embryo25. Cells are colored according to cell annotations from scRNA-seq data25 (b) and unsupervised clustering results from Spatial-DMT (c). d, Spatial mapping of DNA methylation, RNA expression levels, and in situ hybridization (ISH) images from the Allen Developing Mouse Brain Atlas55 show the spatial distribution of selected markers in clusters W7 and W11. e, GO enrichment analysis from one-sided hypergeometric test for differentially expressed genes in clusters W7 and W11. The dot plots show the top GO terms for biological processes enriched in each cluster. The x-axis represents the GeneRatio, and the y-axis lists the GO terms. The size of the dots indicates the count of genes associated with each term, and the color represents the adjusted p-value (p.adjust). f, Spatial distribution of olfactory epithelial cells and olfactory sensory neurons revealed by cell type decomposition of spatial transcriptomic pixels using a scRNA-seq reference25. g, Spatial mapping of DNA methylation and gene expression level of Fstl5 (olfactory sensory neurons marker).
