Extended Data Fig. 9: PTEN loss and MYC cooperate to drive POU2F3+ SCLC. Related to Fig. 4.
From: Basal cell of origin resolves neuroendocrine–tuft lineage plasticity in cancer
a, Survival of RPP and RPM mice treated with naphthalene + K5-Cre three days later versus RPP mice infected with Cgrp-Cre. Number of mice indicated in the figure. Mantel-Cox log-rank test with exact p-values in figure. b, H-score quantification of ASCL1 (top) and NEUROD1 (bottom) in RPP GEMM tumours initiated by indicated Ad-Cre viruses. Each dot is one tumour. Exact tumour number quantified from n = 5–10 mice per group in figure. Error, mean +/- SD. c, Bar graph depicting H-score for POU2F3 IHC from RPP lung tumours grouped by location in the airways and cell type-specific Ad-Cre virus. Each dot represents one tumour. Exact tumour number quantified from n = 4–18 mice/group in figure. Error, mean +/- SD. d, Immunoblot analysis of human SCLC cell line, H1048, for indicated markers after LCV2 infection with non-targeting control (sgNTC) or sgPTEN sgRNAs with HSP90 as a loading control. e, Immunoblot analysis of H1048 for indicated markers in parental cells versus cells with ectopic myristoylated-AKT (myrAKT) with HSP90 as a loading control. For gel source data for (d,e), see Supplementary Fig. 1. Unless otherwise noted, statistics represent Kruskal-Wallis (KW) tests (p-value indicated on figures). If KW was significant, post-hoc Dunn’s pairwise comparisons were performed (exact p-value on figures with ns=not significant=p > 0.05).
