Extended Data Fig. 11: A subset of basal cells targeted by K5-Cre express KRT13 hillock cell marker. Related to Fig. 1.
From: Basal cell of origin resolves neuroendocrine–tuft lineage plasticity in cancer
a-d, Representative tdTom+ cells in naphthalene-injured airway epithelium from Ai9 reporter mice at 3–7 days post-K5-Cre administration stained for indicated basal (KRT5, P63) or hillock (KRT13) markers (upper insets). Bottom panel is merged high magnification co-IF from white box inset indicated in top panel. a, Arrows indicate co-expressing cells in the tracheal epithelium. b, Arrows indicate co-expressing cells in the lung epithelium. c, Arrow indicates tdTom+ cells lacking KRT13 (left) or co-expressing KRT13 in a KRT13+ “hillock” structure (right) in the tracheal epithelium. d, Arrows indicate co-expressing cells in the lung epithelium. e, Percent of tdTom+ cells in the trachea vs lung airway vs total (all airway epithelium) co-expressing or within two cells distance from KRT5 (green), P63 (purple), or KRT13 (turquoise) cells. Quantification reflects all detected tdTom+ cells in mouse airways 3–7 days post K5-Cre administration (n = 4 mice, 160 tdTom+ cells were analyzed for KRT13/tdTom co-stain; n = 90 tdTom+ cells were analyzed for KRT5/P63/tdTom co-stain). Scale bars, 50 µm.
