Extended Data Fig. 5: Adipose-specific ACOX2 deletion impairs thermogenesis and promotes diet-induced obesity.
From: Peroxisomal metabolism of branched fatty acids regulates energy homeostasis
a, Gene expression analysis of ACOX1, ACOX3 and FASN in BAT of ACOX2-AKO and control mice. N = 4. b, Volcano plot depicting Log2FC values of fatty acids in BAT of ACOX2-AKO and control mice. N = 5. c, Total serum bile acids in ACOX2-AKO and control mice. N = 9. d, Core body temperature of ACOX2-AKO and control female mice after cold exposure (4 °C). N = 11. e, VO2 of female ACOX2-AKO (N = 6) and control mice (N = 7) after CL316,243 treatment. f, Body weight of HFD-fed ACOX2-AKO and control female mice. N = 8. g, Histologic analysis of H&E-stained adipose tissue sections from HFD-fed ACOX2−/− and WT male mice. Scale bar, 250 mm. h, Cumulative energy intake of ACOX2-AKO (N = 5) and control mice (N = 4). i, Locomotor activity of ACOX2-AKO (N = 5) and control mice (N = 4). j, Western blot analysis of insulin-stimulated AKT phosphorylation in BAT of liver of HFD-fed ACOX2-AKO and control mice. The bar graph shows quantification of AKT phosphorylation (N = 2). Data with error bars are reported as the mean ± SEM. Data in a-f and h-j are from biologically independent samples. Images in g are representative of 3 mice/genotype. Two-sided unpaired Student’s t test in a and c; two-sided unpaired Student’s t-test adjusted for multiple comparisons using the Benjamini-Hochberg method in b; two-way ANOVA with Fisher’s LSD in d-f.
