Fig. 4: Adipose-specific ACOX2 overexpression promotes thermogenesis and counteracts diet-induced obesity and insulin resistance.

a, Schematic diagram of a transgene construct used to generate adipose-specific ACOX2 overexpression (ACOX2^Adipo-OE) mice. b, ACOX2 gene expression in adipose tissue depots of ACOX2^Adipo-OE and WT mice. n = 4. c, Core body temperature of ACOX2^Adipo-OE and WT mice after cold exposure. n = 10. d, VO₂ of ACOX2^Adipo-OE and WT mice after CL316,243 treatment. n = 5. e, Western blot analysis of ACOX2 in BAT of ACOX2^Adipo-OE and WT mice. n = 2. f, Body weight of HFD-fed ACOX2^Adipo-OE and WT female mice. n = 7. g, Body composition of HFD-fed ACOX2^Adipo-OE and WT female mice. n = 7. h, Haemotoxylin and eosin staining in adipose depots of HFD-fed ACOX2^Adipo-OE and WT female mice. i, GTT analysis in HFD-fed ACOX2^Adipo-OE and WT female mice. n = 9. j, ITT analysis in HFD-fed ACOX2^Adipo-OE and WT female mice. n = 8. Data in b–g,i,j are from biologically independent samples. Images in h are representative of three mice per group. Data are reported as the mean ± s.e.m. Two-sided unpaired Student’s t-test in b,g or two-way ANOVA followed by Fisher’s least-significant difference test in c,d,f,i,j. Scale bars, 75 μm.

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