Extended Data Fig. 6: Astrocytic responses to head trauma.
From: Repeated head trauma causes neuron loss and inflammation in young athletes
a. UMAP representing 4 astrocytic subtypes. b. UMAP from (a) colored by pathological group. c. Stacked bar plots showing astrocyte subtype distribution across pathological groups, statistics performed by chi-squared test. Tests were two tailed. n = 28 individuals. d. Bar plots showing astrocyte subcluster distribution in control and RHI-exposed samples, dots represent individual donors colored by pathological group identity. Bars represent mean, error bars represent standard error of the mean. Statistical analysis was performed using two-tailed Mann Whitney U-test. n = 28 individuals. e. Stacked bar plots showing pathological distribution across astrocyte subtypes. f. Violin plots showing Celda module expression across astrocyte subtypes. Black bar showing median statistic. Colored by astrocyte subtype most associated with specific module expression. Statistical analysis performed by linear mixed effects model. Tests were two-tailed. n = 28 individuals. g. Gene ontology analysis of astrocytic subtypes performed by Metascape. Statistics generated with GSEA and single-tailed hypergeometric test with Benjamini-Hochberg multiple hypothesis correction. n = 28 individuals. h. Dot plot representing expression of selected DEGs across astrocytic subtype and annotated by function. i. Projection of current astrocytic modules on to Visium spatial transcriptomic data. Top row of heatmaps show expression of white matter (PLP1, MBP) and grey matter (SLC27A7, SNAP25) genes. Dotted line indicates separation of grey and white matter. Heatmaps on the bottom row show expression of astrocyte subtype modules based on significantly upregulated genes in each subtype.
