Extended Data Fig. 7: Vascular cell subtype identification and proportion analysis.
From: Repeated head trauma causes neuron loss and inflammation in young athletes
a. UMAP showing all vascular cells colored by Seurat clustering. n = 28 individuals. b. Heatmap depicting vascular cell marker expression. c. Heatmap depicting Jaccard scoring of vascular cell Seurat cluster DEGs compared to Sun and Akay et al. vascular subtype DEGs. Significance denoted by **p < 0.01, ***p < 0.001. d. Bar plots depicting pathological group proportions of vascular subtypes, bar represents mean, error bar represents SEM, dots represent individual samples. Statistical analysis performed by ANOVA with Bonferroni correction. Tests were two-tailed. n = 28 individuals. e. Heatmap depicting Jaccard scoring of vascular cell subtype DEGs compared to Sun and Akay et al. vascular subtype DEGs. Significance denoted by ** p < 0.01, *** p < 0.001. f. Scatter plot of fibroblast proportion or Cap2 proportion compared to years of football play from snRNAseq dataset, colored by pathological group status. n = 28 individuals. Statistical analysis performed by linear regression with age as a covariate. Black line represents linear model regression, grey shows 95% confidence interval. Tests were two-tailed. g, h. Bar plots of total capillary and relative endothelial cell subtype distribution across control and RHI-exposed samples, dots represent individual donors and are colored by pathological group identity. n = 28 individuals. Bar indicates mean, error bars indicate standard error of the mean. Statistical analysis was performed by two-tailed Mann-Whitney U test. i. GO enrichment analysis of DEGs from depicted comparisons. Statistics generated with GSEA and single-tailed hypergeometric test with Benjamini-Hochberg multiple hypothesis correction. n = 28 individuals. j. Proportion of cap2 cells in each individual compared to years of football. Statistics generated with linear regression correcting for age. Black line represents linear model regression, grey shows 95% confidence interval. Each dot represents a single individual. Tests were two-tailed. n = 28 individuals.
