Extended Data Fig. 8: Effector molecule granzyme B but not inhibitory receptor PD-1 is enriched in insoluble aggregates of Tex cells.
From: Proteotoxic stress response drives T cell exhaustion and immune evasion
a, Schematic diagram of fractionate soluble and insoluble proteins in the cell lysates of Teff and Tex cells 8 days post initial activation in vitro for mass spectrometry analysis. Created in BioRender. Wang, Y. (2025) https://BioRender.com/n0ibmgq. b, Representative chromatograms of the retention time and intensity for peptides indicative of granzyme B in insoluble and soluble protein. Raw intensity is on the y-axis and retention time in minutes is on the x-axis. Each colored line represents a fragment ion associated with the peptide for granzyme B. Solid lines indicate fragment ions used for quantitation. Dotted lines represent interfering ions that were excluded for quantitation. The plot on the far right showed the fold change of granzyme B in the insoluble over soluble fractions in Tex and Teff cells. ΔΔ: Tex[Log2FC(insoluble/soluble)] - Teff[Log2FC(insoluble/soluble)]. c, Representative chromatograms of the retention time and intensity for peptides indicative of PD-1 in the insoluble and soluble fractions. The fold changes of PD-1 in the insoluble over soluble fractions in Tex and Teff cells are also shown.
