Extended Data Fig. 2: cGAS-STING-dependent signalling upon MGME1 loss.

a, Agglomerative heat map showing the distribution of non-ISG inflammation related mRNA intensities in kidneys of 55-week-old Mgme1^−/− and Mgme1^−/−; Sting1^mut/mut mice obtained by NanoString profiling. Each column represents a different mouse (left panel). Heat map (log₂ fold change, decreasing order) of mRNA non-ISGs profiles obtained by NanoString technologies (right panel). Asterisks mark ISGs whose expression was significantly altered. n = 6 mice per genotype. b, Agglomerative heat map showing the distribution of mRNA ISGs intensities in Mgme1^−/−, Sting1^mut/mut and Mgme1^−/−; Sting1^mut/mut primary MEFs obtained by NanoString profiling. Each column represents a biological replicate (left panel). Heat map (log₂ fold change, decreasing order) of mRNA ISGs profiles of Mgme1^−/−, Sting1^mut/mut and Mgme1^−/−; Sting1^mut/mut primary MEFs obtained by NanoString profiling (right panel). Asterisks mark ISGs whose expression was significantly altered. n = 3 clones per genotype. q values were calculated using unpaired two-tailed Student t-test with multiple comparisons correction using the two-stage step-up method of Benjamini, Krieger and Yekutieli. c, Agglomerative heat map showing the distribution of non-ISG inflammation related mRNA intensities in Mgme1^+/+, Mgme1^−/−, Sting1^mut/mut and Mgme1^−/−; Sting1^mut/mut primary MEFs obtained by NanoString profiling. Each column represents a biological replicate (left panel). Row-clustered heat map (log₂ fold change) of mRNA non-ISGs profiles of Mgme1^−/−, Sting1^mut/mut and Mgme1^−/−; Sting1^mut/mut primary MEFs obtained by NanoString technologies (right panel). Asterisks mark ISGs whose expression was significantly altered. n = 3 clones per genotype. *q < 0.05, ** q < 0.01.

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