Extended Data Fig. 6: Intra-islet macrophages are the primary cells engulfing apoptotic β-cells.
From: Efferocytic remodelling of pancreatic islet macrophages by limited β-cell death
a. Representative confocal images of the pancreatic islets of NOD mice 12 h after STZ1-low or control vehicle treatment. Green – apoptotic cells (cleaved caspase-3, CC3), blue – nuclei (Hoechst), red – CD11c, white – F4/80, yellow – insulin. Scale bars: 20 µm (left, whole-islet view); 2 µm (right, zoomed-in view). b. Quantification of the fraction of MФ and DC among all islet cells (top), and absolute number of each cell type per islet (bottom). c. Quantification of the fraction of MФ and DC containing apoptotic remnants (CC3+insulin+) among all islet cells (top), and absolute number of MФ and DC with internalized apoptotic material (bottom). Scale bars: 20 µm (left, whole-islet view); 2 µm (right, zoomed-in view). Statistics - paired t-test; N = 2. The diagram in a was created in BioRender. Ravichandran, K. (2025) https://BioRender.com/lwltf25.
