Extended Data Fig. 5: Different R-MCDs, but not other LCDs, drive selective GA-multivalent mRNA retention in nuclear speckles.
From: Collective homeostasis of condensation-prone proteins via their mRNAs
a-c, Example images showing HCR-FISH signal for PSAP (control) or EIF3A (GA-multivalent) mRNAs, with SC35 immunofluorescence in cells expressing different R-MCDs (LUC7L3221–415, PRPF38B251–534 and SRSF11171–389) fused to mGreenLantern. d, Quantification of enrichment of HCR-FISH signal within nuclear speckles compared to the nucleoplasm for cells expressing varying amounts of the R-MCDs shown in panels a-c. e-g, As in panels a-c, but showing EIF3A HCR-FISH signal for cells expressing either an R-MCD with GA-rich GeRM (LUC7L3221–415) or a LCD associated with a different GeRM signature (C-rich GeRM, Pro-rich: mGreenLantern-PRR36832–1099; GC-rich GeRM, Ala/Glu/Gly-rich: mGreenLantern-SIRT12–151). Pairwise significance testing are FDR-corrected Welch t-tests, where * = p < 0.05, ** = p < 0.01, *** = p < 0.001. Precise p-values are found in Source Data
