Extended Data Fig. 6: Expanded characterization of the ΔLTR5Hs hnPSCs.
From: A human-specific regulatory mechanism revealed in a pre-implantation model
a. Bright field images of ΔLTR5Hs hnPSCs with the genes affected by each deletion indicated above each image. Black bar represents 200 µm. These images are representative of the cells morphology in culture, n = 2 biological replicates. b. RT-qPCR results of genes unaffected by LTR5Hs deletions at gene loci shown in Fig. 3b. Plots display the expression of the indicated genes in wild-type or ΔLTR5Hs hnPSCs. RNA values are normalized relative to RPL13A. Above each plot, schematic depiction of the locus is shown. Blue rectangles indicate genes, pink indicates the closest LTR5Hs element, the number on top of the dashed arrows displays distance from the promoter to the LTR5Hs. Grey dots represent expression values obtained in each clone, green, orange, and red squares represent median values. N (independent clones) per gene: GGT5: WT = 5, heterozygous=2, homozygous=5; ZNF98: WT = 5, heterozygous=4, homozygous=2; WRNIP1: WT = 5, heterozygous=4; SULT6B1: WT = 5, homozygous=4; SNHG31: WT = 5, homozygous=2; FAM230I: WT = 5, heterozygous=6, homozygous=6. Unpaired two-tailed Student’s t-test, asterisk color indicates homozygous (red) or heterozygous (yellow), specific p-values included as Source Data, ns: not significant.
