Fig. 2: Antibody profiles illustrate the broad cross-reactivity in A(H5) antigenic space of ferret sera raised by vaccination with whole-inactivated vaccines containing mutated HAs.
From: A vaccine central in A(H5) influenza antigenic space confers broad immunity
a–d, For each vaccine, IraqVACC (a), CVA-VietnamVACC (b), CVA-IndonesiaVACC (c) and CVA-AnhuiVACC (d), antibody profiles are displayed in the antigenic map from Fig. 1b. Each plot displays the mean titres for two vaccinated ferrets. The lines connect the position of the serum with the antigens against which a titre above the assay’s detection limit was observed (that is, >5 for mean serum, ≥10 for individual serum). Antigens and connecting lines are coloured by the HI titre as indicated on the right. The numbers at the top of each panel indicate the distance of the mean serum position to the centre of the antigenic map in antigenic units (AU), the overall geometric mean titre (GMT) of the mean serum and the group mean of the number of titres above the detection limit per serum. The four antigens that were not titrated against these sera are shown in transparent white. One CVA-AnhuiVACC-vaccinated ferret reached humane end points during the study because of a malignant lymphoma, which was unrelated to the experimental procedures. Owing to the premature euthanasia of this ferret, data of a single serum were used for analysis and visualization in d.
