Extended Data Fig. 8: Developmental trajectories of visual cortex Glia cell types.
From: Continuous cell-type diversification in mouse visual cortex development
(a) Transcriptomic trajectory tree for glia clusters starting from the common RG antecedent. Nodes are clusters subdivided by synchronized age bins, and edges represent antecedent-descendant relationship between adjacent nodes, with thicker end at the antecedent node and thinner end at the descendant node. Nodes are grouped by subclass, and adult clusters are labeled. (b-d) UMAPs for glial cells colored by subclass (b), cluster (c) and synchronized age bin (d). (e) Clusters are grouped together based on similar trajectories. Within each cluster group, all cells along their trajectories, including all antecedent nodes, are shown and are colored by cluster membership. (f) Spatial distribution of astrocyte clusters in P56 MERFISH data, based on the ABC-WMB Atlas14. (g) Marker genes illustrating cell type diversification along trajectories. (h) Cluster composition of all glial cells at each age. Specifically, Notch ligands Dll1, Dll3 and Ascl1 are expressed transiently and downregulated as the cells transition from glioblasts to OPCs, while Erbb4 maintains its expression. It has recently been shown that Notch signaling plays a dual role in both promoting and inhibiting oligodendrogenesis to fine-tune regulation of oligodendrocyte generation96. Sox9, strongly expressed in RG and glioblasts, is downregulated in OPCs and turned off completely after cells exit the OPC stage; in contrast, Sox10 is activated at the end of glioblast stage and remains active throughout the developmental process of oligodendrocytes (g).
