Extended Data Fig. 2: Detailed scRNA-seq and Multiome data analysis workflow.
From: Continuous cell-type diversification in mouse visual cortex development
(a) Adjacent cell type mapping and clustering pipeline. (b) The k-nearest neighbor (k-NN) algorithm implementation for building trajectories. (c) Trajectory of glutamatergic cells built from Monocle3, showing that the embryonic part of the trajectory looks reasonable, but the postnatal part of the trajectory appears erratic. (d) Confusion matrix of the fraction of shared cells between each actual age and synchronized age. Developmental ages that were difficult to discriminate in the transcriptomic space were further merged into synchronized age bins for some analyses. Boxes denote synchronized age ins.
