Extended Data Fig. 3: Effects of oestrous state on parental interactions and food intake.
From: Integration of hunger and hormonal state gates infant-directed aggression
a, b, Switching rate does not correlate with P4 (a) or E2 (b) plasma concentration (P, O, M, D; n = 30, 19, 40 and 37 mice). c, Percentage of spontaneously parental virgin female mice in different estrous states before food deprivation (n (left to right) = 35, 26, 52 and 45 mice). d, Attack latency of Agg+ mice in different estrous states (n (left to right) = 17, 6, 29 and 16 mice). e,f, 1-h food consumption in sated mice before (e) and after (f) food deprivation depending on estrous state (Pre: n (left to right) = 10, 3, 11 and 14; Post: n (left to right) = 14, 9, 17 and 20). g, Correlation between total parenting time (see Methods) and number of GFP-labelled MPOA neurons in PgrloxP mice injected with an AAV co-expressing GFP and Cre (linear regression, R2 = 0.581; P = 0.01, n = 10). h, Correlation between attack latency and number of GFP-labelled MPOA neurons in Esr1loxP mice injected with an AAV co-expressing GFP and Cre (linear regression, R2 = 0.113; P = 0.377, n = 9). P, proestrus, O, oestrus, M, metestrus, D, diestrus. Statistics: Fisher’s exact test in c (two-sided, Benjamini-Hochberg adjustment). One-way ANOVA in d–f. Data are mean ± s.e.m. Box plots: median (line), interquartile range (box), whiskers, 1.5× IQR. *P < 0.05. See Supplementary Table 3 for further details of the statistical analyses.
