Extended Data Fig. 8: Dynamic progression of EpoB-induced axon regeneration over hours.
From: In situ structural mechanism of epothilone-B-induced CNS axon regeneration
a) Live images of an EpoB-induced, regenerating axon tip shown at different time points during repair. For about 2 h, the regenerating front displays a flat rapidly moving axon tip. After two hours, a typical actin-rich finger-like growth cone appears. Scale bar: 10 µm. Zoomed-in images of the tip are shown in the insets. Scale bar: 5 µm. b) Live images of EpoB-induced regenerating axons recorded continuously for 6 h. Straightened axon snapshots at different time points are shown for comparison with the axotomy site indicating with a dotted white line. Scale bar: 10 µm. c) Quantification of the cumulative length of axons during EpoB-induced regeneration over 6 h. n = 2 axons from two biologically independent experiments. The bar graph is overlaid with corresponding data points. d) Snapshots of EpoB-induced regenerating axons captured at 0, 24 and 48 h. Images were straightened for comparison. EpoB-induced axonal growth continues for at least 48 h beyond axotomy. Scale bar: 10 µm. e) Quantification of the cumulative length of axons during EpoB-induced regeneration. The bar graph shows mean ± s.e.m. Significance was tested using One way ANOVA. P-values are 0.0030 (0 h vs 24 h) and <0.0001 (0 h vs 48 h). n = 5 axons from three biologically independent experiments.
