Fig. 4: Spatial tri-omics mapping of the LPC mouse model brains at 5, 10 and 21 d.p.l.
From: Spatial dynamics of brain development and neuroinflammation
a, CODEX images of whole mouse brains at 5, 10 and 21 d.p.l. The images at the bottom are magnified views of the regions indicated by the white rectangles. Scale bars, 800 μm (top) and 200 μm (bottom). b, Seurat clustering of the CODEX images in a. c,d, UMAPs and spatial distribution of RNA (c) and ATAC (d) clusters in spatial ARP-seq (S1 and S2). e, Integration of RNA and ATAC data in spatial ARP-seq (S1 and S2). Scale bars, 800 μm. f, The spatial distribution of RNA (left) and H3K27me3 (right) clusters in spatial CTRP-seq (S1). g, Integration of H3K27me3 and RNA data in spatial CTRP-seq (S1). Scale bars, 800 μm. h,i, Spatial mapping of GAS, gene expression and ADT protein expression in spatial ARP-seq (h), and CSS for H3K27me3 in spatial CTRP-seq (i) for Olig2, Mog, Ptprc and Csf1r.
