Fig. 5: CD11c⁺ microglia emerge in distal WM regions.

a–d, Spatial mapping of GAS (a), gene expression (b), ADT protein expression (c) and CSS (d) for Itgam, Itgax and Cd68 in both spatial ARP-seq (a–c) and spatial CTRP-seq (d). e, Schematic of the primary lesion and distal WM of the SM locations in a coronal tissue section. Fx, fornix. f, CODEX images of the SM of 5 and 21 d.p.l. mouse brains. pos., positive. g, CODEX image of 10 d.p.l. coronal section showing CD11c⁺ cells in both the primary lesion and SM. h, Higher-magnification CODEX images of microglia in the SM at 10 d.p.l. i, CODEX image of the LPC mouse brain on a sagittal tissue section at 5 d.p.l. j, Magnified image of the region of interest indicated by a white dashed rectangle (i) in i. k, Magnified image of the region of interest indicated by a white dashed rectangle (ii) in i. l, Magnified image of the region indicated by a white dashed rectangle (iii) in k, with staining for CD4 and CD8. m,n, TRIC-DISCO results of Csf1r from the anterior (m) and posterior (n) direction relative to the injection. Scale bars, 20 μm (h (bottom) and l), 50 μm (j and k), 100 μm (f and h (top)), 800 μm (g) and 1 mm (i). CB, cerebellum; HYP, hypothalamus; MB, midbrain; MED, medulla; OFB, olfactory bulb; P, pons; STR, striatum; TH, thalamus.