Extended Data Fig. 7: Proteomic quantification of DNA repair proteins and CIRBP expression across species.
From: Evidence for improved DNA repair in the long-lived bowhead whale
a, Heatmap of LC–MS quantification of DNA repair proteins in primary fibroblasts. Color intensity indicates log10-transformed ion intensities. b, CIRBP protein abundance in whole liver across mammalian species measured by LC–MS (n = 12 per species; 3 biological × 4 technical replicates). N.D., not detected. c, CIRBP protein abundance in nuclear liver extracts measured by LC–MS (n = 3 biological replicates per species). d, Western blot of CIRBP in fibroblasts from three individuals per species using monoclonal and polyclonal antibodies (mAb, pAb). Experiment repeated three times with similar results. e, Western blot of CIRBP in liver and other organs from bowhead whale and mouse. Experiment repeated three times with similar results. f, Western blot of CIRBP in fibroblasts from multiple mammalian species. Experiment repeated three times with similar results.
