Extended Data Fig. 10: CIRBP promotes DNA repair and survival following DNA damage in human fibroblasts.
From: Evidence for improved DNA repair in the long-lived bowhead whale
a, Lentiviral overexpression of CIRBP variants enhances end-joining frequency in GFP-based NHEJ assays (n = 3 independent experiments). Western blot shows CIRBP expression in human and bowhead whale fibroblasts. Data are mean ± SD; Welch’s two-sided t-test. b, Frequency of insertions/deletions >20 bp in NHEJ reporter constructs from human fibroblasts ± bwCIRBP overexpression, quantified by Nanopore sequencing. c, Distribution of PTEN alleles in fibroblasts overexpressing luciferase or bwCIRBP after CRISPR-induced DSBs (n = 3 independent experiments). Data are mean ± SEM; significance indicated by asterisks (****P < 0.0001, two-way ANOVA with Fisher’s LSD). d, Same analysis as b, performed in bowhead whale fibroblasts transfected with control or CIRBP siRNAs. e, Hypothermia (33 °C) increases NHEJ frequency in primary fibroblasts (n = 3 independent experiments). Western blot shows CIRBP induction under hypothermia. Data are mean ± SD; Welch’s two-sided t-test. f, MTT survival assay after bleomycin in fibroblasts overexpressing bwCIRBP (n = 3 independent experiments). Data are mean ± SD; Welch’s two-sided t-test. g, MTT survival assay after etoposide in fibroblasts overexpressing bwCIRBP (n = 3 independent experiments). Data are mean ± SD; Welch’s two-sided t-test. h, Micronucleus formation after I-SceI cleavage. ≥150 binucleated cells were scored per replicate; bars show individual experiments. i, Chromosomal aberrations in fibroblasts ± CIRBP overexpression after 2 Gy γ-irradiation. 100 metaphase spreads were analysed per sample.
