Extended Data Fig. 3: Bowhead whale fibroblasts show reduced mutagenesis after genotoxic stress.
From: Evidence for improved DNA repair in the long-lived bowhead whale
a, ENU-induced mutational load measured by SMM-seq in fibroblasts from mouse (n = 6 cell lines), cow (n = 7), human (n = 8), and bowhead whale (n = 7). ΔSNV frequency was calculated for each line. Statistical significance was assessed using one-way ANOVA (two-sided, P = 0.0100). b, Mutational spectra showing the ENU-induced signature, including increased A > T transversions (orange) in treated mammalian cells. Same sample numbers as in a. c, HPRT mutagenesis assay after 3 h ENU treatment (n = 3 independent cell lines per species). Mutation frequencies were adjusted for plating efficiency. Data are mean ± SEM.; Welch’s two-sided t-test. d, HPRT mutagenesis assay after 3 h MNNG treatment (n = 3 independent cell lines per species), adjusted for plating efficiency. Data are mean ± SEM.; Welch’s two-sided t-test. e, HPRT mutagenesis assay after 3 h EMS treatment (n = 2 independent cell lines per species), adjusted for plating efficiency. No statistical tests were applied because n < 3. f, HPRT mutagenesis assay after 2 Gy γ-irradiation (n = 2 independent cell lines per species), adjusted for plating efficiency. No statistical tests were applied because n < 3.
