Extended Data Fig. 2: Spatiotemporal accumulation of FD and 14-3-3 proteins at the shoot apical meristem of Arabidopsis.
From: Florigen activation complex forms via multifaceted assembly in Arabidopsis
a, Heatmap showing the Z-score normalized expression values of GRF1 to GRF10, FD and FD target genes in shoot apical meristems dissected from Col-0 plants grown for 7, 10, 13 and 16 long days (LDs). Data are means of three independent experiments for RNA sequencing20. b,c,d,e, Confocal images of shoot apical meristem cells co-expressing gFD::mVenus-FD and either gGRF7::mScarlet-I-GRF7, gGRF8::mScarlet-I-GRF8, gGRF2::mScarlet-I-GRF2 or gGRF6::mScarlet-I-GRF6 at 7, 10, 13 and 16 LDs. The central panels show the expression pattern of the individual chromophores at 13 LDs. The cell walls (blue) were stained with Renaissance 2200. Scale bars = 20 μm. The confocal images in b–e are representative of three independent transgenic lines.
