Extended Data Fig. 4: Gel-filtration analysis of interaction between FT-TwinStrep (FT-TS) and 14-3-3 proteins and the interaction interface between 14-3-3 and FT is highly conserved in different species.
From: Florigen activation complex forms via multifaceted assembly in Arabidopsis
a–d, Size-exclusion chromatography and gel analysis of direct interaction between GRF2 (14-3-3ω), GRF6 (14-3-3ƛ), GRF9 (14-3-3μ), GRF10 (14-3-3ε) and FT proteins. e and f, Alignment of FT and 14-3-3 proteins in Arabidopsis and some other species, respectively. At: Arabidopsis thaliana, Sl: Solanum lycopersicum, St: Solanum tuberosum, Gm: Glycine max, Pt: Populus trichocarpa, Os: Oryza sativa, Ta: Triticum aestivum, Hv: Hordeum vulgare. Sequences can be found in Supplementary Table 1. g, Structural alignment of FT − 14-3-3 complexes from rice (PDB: 3AXY) and Arabidopsis (predicted by Alphafold2). h, Size-exclusion chromatography and gel analysis of MBP-FDT282E, FT, DNA and GRF7 mutant proteins. i, Size-exclusion chromatography and gel analysis of SEP3 DNA (24 bp). j,k, Gel-shift analysis of the interactions between single MBP-FDcT282E WT or combination of GRF7 and FT WT or mutant proteins with SEP3 WT (j) and LFY WT (k) DNA probes, respectively.
