Extended Data Fig. 6: Abundance levels of PE-installed sup-tRNAs compared to wild-type tRNAs.
From: Prime editing-installed suppressor tRNAs for disease-agnostic genome editing
(a) Targeted tRNA-seq of tRNA-Leu-TAA-1-1 measures the abundance of engineered sup-tRNA, ac-only sup-tRNA, or wild-type tRNA at the DNA and RNA levels. (b) Sequence logo of targeted tRNA-seq for reads corresponding to the wild-type tRNA, ac-only sup-tRNA, and engineered sup-tRNA at the RNA and DNA levels. (c-d) Probability of a G base at position 26 (c) or a G base at position 37 (d) for reads corresponding to the engineered sup-tRNA, ac-only sup-tRNA, or wild-type tRNA. (e) Schematic of a Leu-TAA tRNA and the position of its modified bases. The Induro RT used for cDNA generation will result in an “N” nucleotide at any position that is modified in the RNA.
