Extended Data Fig. 2: Endogenous tRNA genes can be converted to TAG sup-tRNAs with prime editing.
From: Prime editing-installed suppressor tRNAs for disease-agnostic genome editing
(a) Comparison of fold enrichment between TAG and TGA stop codon reporters. Values and error bars reflect mean ± s.d. of n = 2 independent biological replicates. (b) Validation of top-performing epegRNAs in an arrayed transfection format. (c) Sequencing reads with the specified edit for 16 epegRNAs validated individually from the TAG PE2 screen. (d) Fold enrichment in GFP-sorted cells compared to plasmid pool for arginine, serine, leucine, and tyrosine tRNA isoacceptors. (e,f) Fold enrichment in GFP-sorted cells compared to plasmid pool for epegRNAs targeting the endogenous tRNA-Leu-TAA-1-1 (e) and tRNA-Ser-AGA-4-1 (f) genes with the indicated spacers, RTT homology lengths, and PBS lengths. Values and error bars reflect mean±s.d. of n = 2 independent biological replicates.
