Extended Data Fig. 8: Comparison of human and mouse constitutively active MLKL mutants for their requirements for SIGLEC12 to induce PMR.
From: SIGLEC12 mediates plasma membrane rupture during necroptotic cell death
a, Cell lysates and SIGLEC12 immunoprecipitation samples from MEFs with CRISPR-induced knockout of SIGLEC12 were immunoblotted with the indicated antibodies. b, Cells were treated with TSE for 3 h, and cell death (CellToxGreen) or cell viability (CellTiterGlo) was quantified. c, Cells were transiently transfected with constitutively active mouse MLKLQ343A or human MLKLQ356A mutants for 24 h. Data were plotted as the mean ± s.d., representative of n = 9 (b, left and c) or n = 12 (b, right); three independent experiments. Statistical analyses were performed using two-tailed t-test. Data are representative of three independent experiments (a).
