Extended Data Fig. 9: Biological processes regulated by OSR2 and TWIST1.
Related to Fig. 2. A. qPCR quantification of selected FAS marker genes, as well as TFs TWIST1 and OSR2 in OSR2 and TWIST1 overexpressing and control intestinal fibroblasts. n = 5 donors per group. Data are presented as mean values with error bars representing standard error of the mean. P values were calculated using paired two-sided Student’s t-tests. B. Dotplots visualising top most enriched gene ontology (GO) biological process (BP) terms in genes found to be significantly upregulated or downregulated in TWIST1 or OSR2 overexpressing fibroblast when compared to intestinal controls. G. RNA-Seq quantification of TWIST1 expression, as in Fig. 2a, except split axes are shown to visualise an increase in OSR2 overexpressing fibroblasts over controls. Boxplots show the median (centre line), interquartile range (box), and whiskers extending to the most extreme values within 1.5× the interquartile range; individual data points are plotted. N = 5 donors per group. C. Venn diagrams visualising overlapping differentially expressed genes between OSR2 and TWIST1 over expressing fibroblasts. D. Violin plot visualising ACTA2 (α-SMA) gene expression in scRNA-Seq data of fibroblasts from this study. Cells from n = 22 donors.
