Extended Data Fig. 3: Mitochondria reduction induced by decreased cytosolic AcCoA is dependent on autophagy.
From: Cytosolic acetyl-coenzyme A is a signalling metabolite to control mitophagy
a-d, RT-qPCR analysis of Mito biogenesis-related gene and nuclear-encoded Mito gene expression levels in indicated cells. Cells treated as indicated for 16 h. n = 3 biological replicates. Data shown as the mean ± s.e.m. Ordinary one-way ANOVA and Dunnett’s multiple comparisons test for all groups except HeLa HSP60 in (b), A549 PPARGC1A and all HSP60 in (c), A549 (NRF1, HSP60) and MCF7 HSP60 in (d) (Kruskal-Wallis test and Dunn’s multiple comparisons test). e,f, ATG7 or FIP200 knockdown blocks SM- and SLC25A1 or ACLY inhibition-induced mitophagy. HeLa cells were transfected with siRNA targeting control (siNC), ATG7 (siATG7), or FIP200 (siFIP200), respectively, followed by indicated treatments for 16 h. Mitophagy was determined by indicated mitochondria markers (e) or qPCR analysis of mtDNA/nDNA (f). n = 3 biological replicates in (e and bottom panel of (f)) and n = 6 biological replicates in (upper panel of (f)). Data shown as the mean ± s.e.m. Two-way ANOVA and Bonferroni’s multiple comparisons test (f).
