Extended Data Fig. 7: Enhanced killing by T cells from clusters.
From: Tumour-reactive heterotypic CD8 T cell clusters from clinical samples
a, Quantification of cytokine production by expanded CD8+ T cells derived from singlets (blue), tumour cell clusters (purple) or APC clusters (green) after a 4 h co-culture with autologous tumour cells (n = 12 patients). Points represent technical replicates. Unpaired t-test versus T cells from singlets was used. Mean ± S.D. b, Tumour killing by expanded CD8+ T cells derived from either singlets, tumour cell clusters or APC clusters (n = 11 patients), normalized to untreated tumour cells. Points represent technical replicates. On the x-axis, the T cell:tumour cell ratios are shown. Two-way ANOVA, followed by Dunnett’s multiple comparisons test was used. Significantly increased killing compared to T cells from singlets from the same ratio is indicated. Mean ± S.D. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.
