Extended Data Fig. 2: Root penetration phenotype of cesa6 mutants in agar media.
From: Ethylene modulates cell wall mechanics for root responses to compaction
a, A phylogenetic tree of the 10 CESA proteins from Arabidopsis and the 11 CESA proteins from rice. AtCESA6 is functionally redundant to CESA2, 5 and 937 implying a similar relationship of OsCESA6 and OsCESA3 and 5. Analysis was performed using MEGA 7.0. Bootstrap values indicate the homology of amino acid sequences. b, Genotypic characterization of CRISPR-Cas9 cesa6 mutant lines revealed a deletion of an ‘A’ in the third exon, resulting in a frameshift mutation and premature termination. Black and white boxes indicate exons and introns, respectively. c-d, Primary root penetration phenotypes of WT, cesa6 mutants, and NG-CESA6 (complementation line of cesa6) seedlings grown on normal (left panel) and dense (right panel) agar media for 5 days. The experiment was conducted in triplicate, yielding consistent trends (n = 10 plants per trial). Statistical analysis was performed using two-way ANOVA followed by Tukey test. Different letters denote significant differences at 0.05 level. The p (interaction) <0.0001. Based on one of three replicates. Box plots show 50th percentile median (centre), 25th-75th percentiles (box), and minimum to maximum range (whiskers) in (d).
